FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7690686
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT29750.065.649439
CGGGATT35800.060.204769
CGCGATT11350.057.9157839
CGGTATT25300.056.7106749
GGGGATT93850.055.1580739
GGGTATT58950.053.0738839
GACGATT13950.053.0116239
GGCGATT28100.051.2847259
ACGGATT14350.051.0934879
GCGGGAT26500.049.61128
GCGTATT26200.049.4552089
CGCGTTA5000.049.3011327
GCGGTAT17200.049.242348
GCGTAAT16450.048.797479
GGCGAAT27150.044.465489
CGTTATT19650.044.0674069
GGCGTAT16700.042.390048
CGCGATA7500.042.1374449
CGGTAAT21750.042.1374449
GGCGGAT29050.041.775098
Produced by FastQC (version 0.11.5)