FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9705798
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5056695.209968309663976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT23950.052.70489
CGCGAGT17550.051.3232089
CGCGATT12650.051.146079
CGCGTTA5850.046.6246077
CGGGATT36750.046.602489
GCGTAAT16300.045.5303349
TGCGAGT23650.043.9860889
CGGTATT23200.043.7456259
GGGGATT122050.042.460679
CGGTAGT22000.041.5185749
CGGGTTA15000.041.0188147
TGCGTTA9600.040.9659547
CGTGATT23600.040.3164149
CGTTAGT14050.039.7291229
CGTTATT21550.039.1477669
GCGGAGT42950.039.1368039
GGCGATT31600.038.1389859
GCGCAAT21750.037.9128769
CGCTAAT11600.037.7306029
GGGTATT71050.037.67489
Produced by FastQC (version 0.11.5)