FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2806153
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1964166.999475794798074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT3250.073.789949
GCGGATT9500.073.6849759
GGGGATT40650.067.127489
CGGGATT11800.066.463339
ACGGATT4650.064.1185469
GGCGATT10100.062.890279
CGTTCGA1600.060.764527
GGGTATT21850.058.141129
GCGGGAT9950.057.9756628
GCGTAAT6100.057.377649
GCGTATT8450.055.994459
GACGATT5000.051.8523869
GTACGGA3000.051.8523867
GGCGTAT5550.051.3852548
GGCGAAT9000.051.1322179
GGTGCGA8750.049.6301427
CGCTAAT3800.049.464459
CGCGAAT4850.049.4468659
GGCGGAT9850.049.351898
CGTCGAC3100.048.0889138
Produced by FastQC (version 0.11.5)