FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2435723
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG54300.2229317537339016No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG40450.16606978708170017No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA34570.14192911098675834No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC34500.14164172198562808No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC32650.13404644124147122No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG31510.129366106080207No Hit
GCAAGTGAGTGGTACAAGAGTGCAGACTCACAGTTTAAATTATTCTCTTA28510.11704943460319585No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA27950.11475032259415377No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA26320.1080582644249777No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT25810.10596443027388583No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC24900.10222837325919246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG850.0107.457341
GTTCGCG1400.0105.3821953
ACCCGTT2700.0104.0854647
TCGCGCT1500.098.3607565
CGATCGT801.909575E-889.1911145
CGTCGAC2150.084.964528
CGCGATA950.081.3517
GTATGCG1150.079.421735
CGCCGTA1700.078.523297
GCTAGCC6700.071.309231
ACGCGTA852.8525192E-669.953804145
CTATGCG2400.067.3302845
CGCGCTT1900.066.561289
TCGTGTT9400.066.519214
CGCACTA852.237357E-1066.1275941
CCGACGT3000.065.573845
CGCGTTC756.2282197E-965.573845
TTCGCGC2250.065.5724954
GACGCGT1001.2732926E-1163.2332089
CTTTCGT10150.062.300011
Produced by FastQC (version 0.11.5)