FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7089453
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTGTTCTCGTCGTTTCCGCAACAAGTCCTCTTCAGAAATGAGCTTTTG175050.2469160878843544No Hit
CGAGCTGCTGTCGTTGAGAGGGTAGGGGAAGACCACCGAGGGGTCGATGC166460.23479949722496218No Hit
ACCCTCTTGGCAGCAGGATAGTCCTTCCGAGTGGAGGGAGGCGCTGCGTA163520.23065249180719585No Hit
ATTTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTA162800.2296368986436612No Hit
GCCAGCTTCTCTGAGACGAGCTTGGCGGCGGCCGAGAAGCCGCTCCACAT154310.2176613625903155No Hit
ACGTCTCCACACATCAGCACAACTACGCAGCGCCTCCCTCCACTCGGAAG148720.20977641011231757No Hit
CTCAGGACATTTCTGTTAGAAGGAATCGTTTTCCTTACTTTTCCTTACGC144230.20344305830083081No Hit
CCTGAGACAGATCAGCAACAACCGAAAATGCACCAGCCCCAGGTCCTCGG137820.1944014580532518No Hit
TAAATAAGCTGCCAATGAAAATGGGAAAGGTATCCAGCCGCCCACTTTTG134760.19008518710822964No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA134190.18928117585376475No Hit
GCATTAATCTGGTAATTGATTATTTTAATGTAACCTTGCTAAAGGAGTGA132460.18684093116916073No Hit
CCGCCTCAGAGTGCATCGACCCCTCGGTGGTCTTCCCCTACCCTCTCAAC129530.182708031211999No Hit
CAACAGGAACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACT125330.17678373775804707No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG120610.17012596035265343No Hit
GCACCAAGACCCCTTTAACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGC113110.1595468648991678No Hit
CGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTCCCAGCAGCTCGG107110.15108358853637932No Hit
ACCTTCATCAAAAACATCATCATCCAGGACTGTATGTGGAGCGGCTTCTC103880.14652752476107817No Hit
GTAATATATTTGGTCTGCGGCCTGTCGATTATACACATCTCCGAGCCCAC103590.1461184664035434No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT86920.12260466357559602No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG83670.11802038887908559No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA82830.1168355301882952No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC77790.10972637804355287No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG74410.104958732359182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCCG19650.0113.77767145
TCACGCA29150.097.78927145
ACCCGTT6600.097.305417
GTTAGCG3550.086.598971
GCGCGCA25750.085.196556145
ACGCGTA3100.081.13541145
TGTCGTT34250.074.7990659
CGATCGT3300.071.98377145
GCATTCG34600.070.492877
ACGTCTC34900.070.2744751
CGAACGT2950.069.4748841
GCTAGCC18000.066.798811
TTACGCG4150.065.850936
ATTCGAC37300.065.752339
CTGTCGT42600.065.591638
CGTCGAC6450.062.492378
CATTCGA40550.060.483698
GCTTAGA40100.058.6089676
GCGGATA4900.057.03396145
CGCCGTA4250.056.264217
Produced by FastQC (version 0.11.5)