FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7089453
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3764315.309732640868061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT26200.076.041579
CGCGATT10600.073.753249
CGCGAGT14700.058.3293159
GACGATT12150.058.1175169
CGGGATT30450.057.3532149
GCGTAAT14650.056.376619
GGCGTAT11800.056.1012548
GCGTATT21900.054.6984189
ACGGATT16550.054.4757659
GGCGGAT25050.053.6089328
GGGGATT94600.053.5167479
GGCGATT31300.053.3785559
CGGTATT19400.052.3226179
GGCGAAT31450.051.720699
GCGCATT19900.051.6416139
GCGCAAT17500.050.797979
GGGTATT53350.049.988639
CGGTAGT18050.049.59949
CGCGAAT11700.049.036659
CCGCATT10950.048.940699
Produced by FastQC (version 0.11.5)