FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7726093
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT30450.064.238239
GGGGATT105100.059.4963159
CGCGATT10250.059.097149
CGGGATT34700.058.916199
GGGTATT58300.055.738829
GGCGATT28450.054.5596929
GCGTATT24550.052.946219
ACGGATT16000.050.478819
CGGTATT27300.050.1552249
GCGGTAT15650.048.3822448
TCGCGAG7600.048.154138
GGCGGAT26300.047.743748
GACGATT13000.047.566579
GCGGGAT29200.045.5951588
GGCGTAT14950.045.582878
CGCGCTA6250.045.4309277
GCGTAAT16500.044.3601659
CGATATT20200.044.044019
GGCGGTA21350.044.035967
CGCGTTA5350.043.6382187
Produced by FastQC (version 0.11.5)