FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5918848
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3115525.263726995523453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT8200.070.782019
GCGGATT17000.059.1058929
CGCGAGT10900.055.5391249
GACGATT10850.054.069459
GGGGATT75150.051.655349
CCGCATT6650.051.617259
CGTTATT11550.051.332869
CGGGATT22500.051.314859
ACGGATT11200.049.5936249
GGCGATT22300.049.2562839
CCCGATT8250.047.6585049
CGGTATT14750.047.3892489
GGCGAAT23600.046.807469
GCGCATT14000.046.8074579
TGCGATT14150.044.105979
GGCGAGT30450.042.4264689
GGTGCGA16600.042.1079147
GGGTATT39700.041.973449
GGGGCGA38700.041.9291657
TGCGAGT14600.041.891619
Produced by FastQC (version 0.11.5)