FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2711593
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1900537.008905835057106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT7750.068.840219
GTACGGA2900.062.8188677
GGCGGAT6250.059.3369068
GCGTATT8200.058.7148449
CGTTATT7450.058.5124939
CGCGTAA2350.058.140867
CGGTATT8300.057.223559
GGGGATT32250.057.093519
CGGGATT9250.056.9735579
CGCGTTA2000.055.303047
ACGGATT4950.055.2044569
CGCGATT3100.054.568469
TCGCGAG1550.054.568468
GGCGATT7600.053.9333049
GGGTATT18550.050.5066579
GCGTTAT3800.049.652888
GGCGTAT5500.048.5010578
GCGGTAT5700.047.9407168
TCGCCGA2150.045.392377
CGCGAAT4050.044.981419
Produced by FastQC (version 0.11.5)