FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6568282
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATTTGGTCTGCGGCCTGTCGATTATACACATCTCCGAGCCCAC164970.25116156705817444No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG116140.17681944837325803No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA92550.1409044252363099No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA77870.11855459311887034No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG76270.11611864411424479No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC72760.11077478098534747No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC71040.10815613580537498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT7800.092.169637
GTTAGCG2150.091.4709551
GCTAGCC18800.071.061121
GTTCGCG4250.068.615623
CGATCGT2100.067.235115145
CGCGTTC4350.060.8053785
CGTCGAC5950.059.2711148
TTCGCGC5300.055.02284
GTACCCG13000.054.7788165
CGAACGT3100.054.6892551
TCGTGTT20900.053.5411574
TTACGCG3300.053.435036
CTAGTCG12850.053.304243
CGCGATA4200.053.289457
ACGCGTA2703.6379788E-1252.293976145
AGTACCC45500.051.868622
CGGCGAT3800.051.7548752
CTTTCGT22350.050.6713641
CGATTAG3750.050.6349561
GCTTAGA32700.049.7771646
Produced by FastQC (version 0.11.5)