FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2992234
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA68490.22889252645347924No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG58800.19650869550977632No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA52060.17398371918773733No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG49020.1638240859504972No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC42790.14300352178338993No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG42270.14126568978228307No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG32460.10848082068447855No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC30080.10052689729479712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT3850.0106.3373647
CGATCGT903.6379788E-12104.6955145
GTTAGCG1300.097.757351
GTTCGCG1850.087.755583
CGTCGAC2500.084.70328
GCGGATA1950.084.56175145
GCTAGCC9800.082.129471
CGAACGT1150.079.811551
TCGTGTT10350.071.610184
ACGCGTA1650.071.38329145
TTACGCG905.456968E-1270.587196
CTTGACG3350.070.31788145
CGTATCG2200.069.59872145
TCGCGCT2450.069.146635
TTCGCGC2350.069.085334
CGCGACT1550.068.3090443
ATTGCGT2650.066.591694
TAGGACG1950.065.156328
GCGTAAC2850.061.917557
TCCCGTA2400.061.7627569
Produced by FastQC (version 0.11.5)