FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2992234
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2101667.023715391242797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT12650.074.422429
CGCGATT4050.073.7444159
CGGGATT12600.071.625989
GGGGATT39800.069.494889
GGCGATT11950.067.37219
CGGTATT9750.063.928269
ACGGATT6700.062.0199559
GCGCATT8100.060.9192969
GGGTATT24100.057.922559
GACGATT5300.057.5768979
GCGTAAT6350.057.2585879
GCGTATT9050.056.6767359
CCGCATT5300.055.1268169
GGCGGAT11300.054.5847668
GCGCAAT7050.052.4942869
TCGCGAG2900.051.493948
GGCGAAT11100.051.4737669
CGGTAGT7650.050.923259
GCGGGAT11000.050.761228
TCGGATT8650.050.290399
Produced by FastQC (version 0.11.5)