FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7027356
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3664605.214763561145899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT22950.072.230679
CGCGATT9700.071.098079
CGGGATT28950.056.6049279
GGCGATT29900.055.6528749
GACGATT9750.055.1591229
GCGTAAT13200.053.2049569
GGCGGAT24550.053.090748
GCGGATA16000.050.6166089
CGCGAGT14500.050.6166049
CGGTAGT18350.050.3407679
GCGGAGT35900.050.2288749
ACGGATT13000.049.6432159
GCGCATT16600.049.168249
CGGTATT20350.048.50249
GGGGATT94000.048.2607779
GGCGAAT28700.048.0593269
GGGTATT51100.047.7935689
GCGGAAT27500.046.9354029
CGCGAAT11750.044.6933869
GGCGAGT44350.044.3679969
Produced by FastQC (version 0.11.5)