FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6497768
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3369565.185719157716926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT19350.067.22329
GCGTAAT11000.061.422019
CGCGATT8850.060.6468939
CGCGAGT14250.060.2638639
CGGGATT30800.058.018789
CGCGACT9400.057.770159
GGCGGAT19250.054.7795758
CGCAGAC28000.053.9006423
GGCGATT28550.053.302049
GGGGATT84550.050.6347859
ACGGATT13400.050.421059
GCGGAGT31300.050.232869
GCGTATT19000.049.850629
GCGGAAT21500.048.459449
CGGGACT19700.048.3998079
GGCGAGT39000.048.248589
GGCGACT27800.048.153079
GGGGCGA52900.047.9847567
CCGCATT9500.047.8565989
GCGGACT21650.047.8320359
Produced by FastQC (version 0.11.5)