FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2804901
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1958736.983241119740055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATT9000.066.911869
GCGGATT10100.064.753429
CGCGTTA1800.064.753417
CGCGATT2500.062.1632779
GGGGATT36700.061.7539379
GCGTATT8550.057.558599
ACGGATT5300.057.422849
CGGTATT8450.055.1745079
CGCGACT4450.053.8399169
GACGATT3850.053.8210149
CGGGATT9900.053.634149
GGGTATT23450.053.569999
GGCGAAT8300.051.4906659
CGATCGA1450.049.123287
GGCGTAT6250.048.694578
CGCGAAT3800.047.7130369
CGGGTAT5850.047.5965278
TCGCGAG1950.046.4896248
AGCGAAT5300.045.205219
CGTTCGA2150.045.17687
Produced by FastQC (version 0.11.5)