FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5649931
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA127660.22594966204012049No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG100080.17713490660328418No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA85930.15209035296183263No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT63550.11247924974658982No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC61190.10830220758448202No Hit
TTCTACCCCACATCCATTGTCTGAAACTTCAATAAGATCCACTCCATAGT56520.10003661991624324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT6100.0102.8213657
ACGCGTA3450.080.22015145
GCTAGCC18150.079.383441
GTTAGCG2300.077.930191
CGATCGT2400.076.87765145
TTACGCG2850.070.1334156
CGAACGT2300.068.938251
GTTCGCG3200.066.767383
CTTTCGT20050.066.703351
CGTCGAC3500.064.985698
TCGTGTT20950.063.1668244
CGCGACT4650.060.7691963
CCGACGT9000.060.500145
TCGCGCT3600.059.3514065
GTACCCG10850.058.4426545
CGTATCG6100.058.33339145
TACCCGT10800.058.0750276
CGCCGTA3450.057.9363027
CTAGTCG11350.057.6873173
TTCGCGC3750.056.977354
Produced by FastQC (version 0.11.5)