FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5649931
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3980397.0450240896747225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATT23300.067.804879
GGGGATT69250.063.8043639
CGCGATT7350.062.0372779
GCGGATT18600.061.459469
CGCGAAT8200.058.739359
GACGATT9900.057.734659
GGCGATT21950.057.346029
CGCGTTA2800.055.0471577
GGGTATT40200.053.9973379
CGGTATT20150.053.5446329
GCGCATT15800.052.02779
ACGGATT11950.051.0548979
GCGGGAT18250.050.673558
CGTTATT14550.049.4352539
GCGGTAT11200.049.3130768
GCGTATT14200.048.8446669
GCGCAAT11950.047.8303769
CGTGCGA6200.046.6125147
CCCGATT8300.044.8778089
GGCGGAT19200.044.152418
Produced by FastQC (version 0.11.5)