FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6594627
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3466905.257158592896914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT21450.070.618849
CGCGAGT13600.066.828279
CGCGATT10800.063.115599
GGCGATT26650.056.3658949
ACGGATT13500.055.6352239
CGGGATT28000.053.873669
GCGTAAT12800.053.7468769
CGCGTTA4200.051.093577
CGCGAAT10400.050.9779789
GACGATT11300.050.82769
GCGCATT17900.050.7745559
GGGGATT81750.050.2608579
GCGTATT21350.049.073489
CGCGCAT7850.049.0452358
GGGTATT46800.048.820189
CGCTAAT8450.048.5504539
GCGGAGT35300.048.454189
GGCGAGT37800.048.255049
CGGTATT20650.046.763619
GGCGGAT22050.045.798168
Produced by FastQC (version 0.11.5)