FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7358154
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT28100.059.3292969
CGCGTTA5300.054.809597
CGCGATT11700.052.354929
GGCGATT26650.051.894169
GGGGATT86400.051.8938879
ACGGATT14050.051.2389379
CGGGATT32450.050.403039
CGGTATT23800.050.1483279
TCGCGAG5550.050.0650068
GGGTATT57400.050.0577249
GGCGAAT25050.048.906399
GCGTAAT16700.048.024069
CGTGATT21750.047.3260159
GCGTATT25950.046.723519
GCGGTAT15150.046.6852768
CGTTATT18400.046.3326959
CGATATT18000.044.2048269
ACGCGAT5150.044.143818
GACGATT10950.042.676549
CGCGATA5600.041.7239389
Produced by FastQC (version 0.11.5)