FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6115261
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA133290.2179628964323845No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA108700.1777520207232365No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG103920.16993551052031958No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG82360.13467945194816705No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG79650.1302479158289401No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC69780.114107966937143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT8400.092.4781957
GTTAGCG2000.088.5771261
GCTAGCC26250.085.3974461
GTTCGCG2800.077.8738253
CTTTCGT29350.077.770511
TCGTGTT29350.076.381354
CGTCGAC5050.075.558278
ACGCGTA2150.066.77853145
TTCGTGT34050.066.6387253
TTTCGTG38200.063.5002672
CTAGTCG10800.061.1994553
CGGCGAT3350.061.019042
GTACCCG12900.060.746095
CGTGTTC37950.059.2532775
CGTATCG4400.058.734745145
GCGTGAT14700.054.235337
CGAGTTA5700.053.7961777
CCGACGT7800.053.2899675
AGCGTGA15000.053.1506276
CGCGATA3350.052.8861927
Produced by FastQC (version 0.11.5)