FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6115261
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3175495.1927301222302695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT8250.063.4047859
GCGGATT19550.060.9274449
CGCGAGT11300.054.656979
CGGGATT24900.051.1269539
GGCGATT23450.048.1069839
GCGTAAT11550.048.017399
GGCGAAT22750.047.9250569
GGGGATT81550.046.832429
CGCGTTA2750.045.8347857
GACGATT9500.045.1110769
CGCGACT7900.043.8766569
GGGTATT42550.043.6938559
CGGTATT15950.043.0688939
CGATCGA2500.042.8555267
GCGGAAT21050.042.813619
GGCGAGT32250.042.7968989
CGGTAGT14000.042.765499
GCGGAGT29450.042.5858849
GCGGGAT20150.042.5365038
CGCTAAT8400.042.015229
Produced by FastQC (version 0.11.5)