FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7658903
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT26450.075.11829
CGGGATT34650.060.7260369
GGGGATT114900.060.582829
GGCGATT28500.057.8537379
GGGTATT64150.056.7826469
ACGGATT20300.054.3753789
CGGTATT19700.053.580119
CGCGATT11700.052.4786349
CGTTATT16300.051.2125179
CGCGAGT15000.050.5917639
CGTGATT20950.048.407399
GACGATT14200.047.611969
GCGCATT23350.045.204639
GCGTATT24350.045.0481039
TGCGATT20950.044.785079
GGGCATT76500.044.188889
GCGGGAT27950.044.1824268
CCCGATT12550.043.9769029
GGCGGAT25400.043.457498
GGCGTAT14100.043.0568168
Produced by FastQC (version 0.11.5)