FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11306698
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG280590.2481626377568411No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA211000.18661504888518293No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA210900.1865266057340525No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG199720.17663866143767173No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT177760.15721654544943184No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG176930.15648246729504936No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC173800.15371419666466726No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC163880.14494063607252977No Hit
GCAAGTGAGTGGTACAAGAGTGCAGACTCACAGTTTAAATTATTCTCTTA148390.13124079196242794No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA141520.1251647474797682No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC138950.12289175849571643No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA126890.11222551446938797No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG121690.1076264706106062No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG114600.10135585119545953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT13200.0105.8359457
GCTAGCC41400.087.561551
CGATCGT4850.085.305115145
GTTAGCG4250.083.816141
CGATTAG7500.081.953561
CGTCGAC13300.080.352558
CGCCGTA6450.079.056997
TCGTGTT46850.076.931084
CCGACGT16150.075.2578665
CTTTCGT49550.071.467851
GTTCGCG5450.069.208123
CGAACGT3700.067.959031
CGCGTAG6400.067.6685646
AGCGTGA30150.066.260386
TTCGTGT56400.063.656663
CGCGCTT8700.063.4257779
ACGCGTA4100.061.157406145
GTACCCG23150.060.3467985
TTTCGTG66300.058.5746352
AGTACCC92650.058.4257932
Produced by FastQC (version 0.11.5)