FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6181902
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA109740.17751818129760064No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG103410.1672786142517303No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA98070.15864049608033257No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC81750.1322408540284204No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA80130.12962030132473792No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT69190.11192348244925268No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA69170.11189112994673808No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG69030.11166466242913589No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG62710.10144127163452282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT7250.0114.293667
GTTAGCG2850.087.2332841
GCTAGCC22400.078.000451
CGCCGTA4000.077.6839757
CTTTCGT25900.066.393291
TCGTGTT26200.065.099044
CGCGATA3950.064.6820147
CGATTAG3650.062.4375231
TTTCGTG32650.060.6984562
ACGCGTA1952.0008883E-1160.520126145
GTACCCG13850.059.8288155
TACCCGT13900.059.6136056
TTCGTGT29350.058.5827983
CGAACGT2250.058.3170621
CGTCGAC7150.056.9798628
ACGGGTC8550.056.533698
GCTTAGA35150.056.3813486
CGATCGT2350.055.7987145
CTAGTCG12950.055.4552273
GTTCGCG3700.054.1221663
Produced by FastQC (version 0.11.5)