FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4393820
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG90610.20622146560396196No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA59510.13544023196216504No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT58050.13211738305164983No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA57480.1308201064222021No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG55120.12544892599150625No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC54400.12381026077536177No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA53750.12233091023300909No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC50280.11443345426075716No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG49720.11315893687042254No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC49370.11236236350146342No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA48610.11063266132886646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTA1450.0146.02208145
ACCCGTT4550.0118.1372457
GTTAGCG2400.0113.467011
CGATTAG2750.083.791031
GCTAGCC11500.068.004311
CGTATCG3650.064.83333145
GTACCCG8250.064.306125
CGCGATA2650.063.222677
CGTCGAC3900.062.6492468
TAGCGGT4350.062.5825423
TCGTGTT16250.061.858844
CGATCGT1850.060.591038145
TCGCGCT1900.058.7839935
CGCGTAG1550.058.5469556
CGTAGCG1001.593071E-858.225773140-144
CGCCGTA1800.058.173647
TACCCGT9150.057.9809276
CGAACGT2000.055.8606871
GTTCGCG2000.055.8428843
CTTTCGT18550.054.5808531
Produced by FastQC (version 0.11.5)