FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4360118
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG81090.18598120509582539No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA77300.17728877979907884No Hit
CTGAGGGAAGGATAATGGTATAGAGAACCTCAAGGTTTTTCTTAGGGTTT58840.13495047611096764No Hit
CAGAGAATATATTTGGTGGCATTTACGAATCACTTCTTCAGGGGACTGAG58130.13332207981527106No Hit
CCAGGCTACCTAGAATTGAACACGTCAGATTATTTTTTCATTTCAATTGT56640.12990474111021766No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA56410.12937723245104835No Hit
GTAATATATTTGGTCTGCGGCCTGTCGATTATACACATCTCCGAGCCCAC55690.12772590099625744No Hit
CAACTGGACAGGTTTCATAGCGGCAGACATTAAAGGTACAGGTTCCTGGA55560.1274277439280313No Hit
GGACAAAAATACAAGATAAACTATTTTATTTTTGGCCCTCAACTTGGACC54490.12497368190493928No Hit
AACAAGTTGCAGGGAAGTCTTAAGAGAGCCGCCCGAAGCCCCGCACCTCC54270.12446910840486426No Hit
CGCAATGACCCCGCACGATTTCATTGAACACTTCCTCTCCAAAATGCCAG52010.11928576244954839No Hit
AGATTGGTATATCTTGTCCAGCAACCATTTTTGTGCCCATGTTTTCTCAT49440.11339142656230865No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG49300.11307033433498817No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC48540.11132726224382002No Hit
GGCTTTACAAACAGCGGTTGACATCTAATATTTGACAAAATATTTTGCTT47660.10930896824352002No Hit
GTCAAAAATGAAATTGACTGAATCAGTGTGTACCCACCAAGTATGTTTTC45830.10511183412925981No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA45170.10359811362903482No Hit
CTTCCTGAAATGTGCTACACTGGACACCAGAGCCCTGCTTAAATTGGCTT45120.10348343783356323No Hit
TTTCCCTTCTGGTATCAAAATGCTCCGGAGAGGAGGGACTGTCAGGGGAG43640.10008903428760416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTA2350.096.677666145
GTTAGCG1400.086.6010061
ACCCGTT5450.085.477857
TCGAATG8100.077.13326145
CTAGTCG7700.076.243783
ACGAAAG12550.072.41196145
GCGTGAT9800.070.978647
CGATCGT1352.812356E-670.12115145
GCTAGCC10250.067.8578341
TCGTGTT16250.066.365244
CGCGATA3350.062.863197
AGCGTGA11300.062.1207286
CTTTCGT17550.061.811481
CGTCGAC4350.061.6108788
TAGTCGA9550.061.474044
AGTCGAA9750.058.906095
GTACCCG7950.058.5973555
TCAAACG13400.057.928444145
TTCGTGT18850.057.888473
CGCGCTT3250.056.938639
Produced by FastQC (version 0.11.5)