FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4360118
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2280635.230661188527466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT13150.067.792829
CGCGATT6650.060.600889
GGCGATT15800.059.1276979
CGCGAGT7450.057.3717889
GGGGATT52500.052.3371249
CGGGATT18600.051.2115949
CGGTATT11400.050.8833129
GCGCATT8900.049.3968359
GGGTATT26750.048.847989
GACGATT6900.048.6709949
CGCTAGT4700.048.068499
CGCGAAT6500.047.9085969
CGTGCGA4650.047.272247
TGCGAGT8700.047.0231979
ACGGATT9000.046.134219
GGCGGAT12350.045.980398
CGCGACT6350.045.1939969
TGCGATT8450.044.8014079
GCGGAGT18750.044.6144879
GCGGAAT18350.044.5887579
Produced by FastQC (version 0.11.5)