FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5491284
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2838595.16926460186725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT15100.066.642559
CGCGATT6800.053.0514689
GCGTAAT11050.052.1207359
CGGGATT21500.051.514689
CGATCGA3400.050.2592857
TGCGAGT14500.049.322149
CGCGAGT9000.048.5219239
CGCGTTA3900.047.0613987
CGGTAGT12850.044.8197759
GCGTATT17500.044.4834569
GGCGATT21800.044.418759
CGCGATA5200.043.8157849
CGAGAGT12000.043.2478039
CGCGTAT5200.042.598688
GCGTTAT7900.042.4600228
GGGGATT66650.042.1613279
GCGGAGT26500.041.794939
CGCGACT8950.041.7215469
CGTTATT11550.041.645029
ACGGATT10950.041.6149949
Produced by FastQC (version 0.11.5)