FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7312666
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT28150.073.863479
CGCGATT15550.072.729729
CGGGATT37250.070.150879
GCGTATT27100.066.3570569
GGGTATT60800.065.854849
GGCGATT37650.065.67419
GGGGATT104700.063.670169
CGGTATT25750.063.561649
GCGCATT29250.059.5582929
ACGGATT18500.057.7149439
GACGATT11850.057.5002569
CGCGTTA5400.054.6351287
GGCGTAT16050.054.2704058
TGCGATT21950.054.0839739
CCGCATT16350.053.7042549
GGGCATT76650.052.512049
CGCGTAT9950.052.2426348
GCGTAAT17150.050.789519
GCGGGAT27400.050.5047388
CGCAGAC34000.048.9693643
Produced by FastQC (version 0.11.5)