FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8135909
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4223735.191466620386241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGT15150.067.204079
GCGGATT26100.065.903849
CGCGATT12150.060.8964429
GACGATT13650.055.5943839
CGGGATT34500.053.1570939
GCGTAAT15300.052.9055069
GGCGATT36200.052.0583089
ACGGATT16800.050.8167469
GGGGATT103550.050.5664639
CCGCATT14000.049.235789
GCGGAGT38900.047.3070379
CGCGACT13200.046.47089
GCGGAAT30250.046.409839
GCGGGAT27850.046.3220068
GCGCAGT29500.046.089169
GCGCAAT18550.046.0227139
CGCGTTA4750.045.2655337
GGCGGAT25100.045.0984578
CGGTATT23900.044.981449
GGCGAAT35550.044.8273749
Produced by FastQC (version 0.11.5)