FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7143796
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3682485.15479445381699No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT22800.068.585269
CGCGATT12250.065.118319
CGCGAGT15050.064.361139
CGACCGA5400.056.2750857
ACGGATT13700.055.915669
GGCGATT29950.055.1711969
GCGTAAT12200.053.44989
GGGTATT54950.053.3435639
GCGCATT19050.052.176319
GGCGGAT21750.051.5208138
CGGTAGT17200.051.1628889
GGGGATT99550.051.1309059
TGCGAGT18300.049.8172849
CGGGATT27550.049.6364639
GCGGAGT37850.049.008399
CGGTATT20050.048.6267289
CGCGCTA5600.048.612637
GACGATT12100.047.089699
GGGGCGA68000.046.551087
CCGCATT11750.046.3371479
Produced by FastQC (version 0.11.5)