FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31820365
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAG110200.057.326291
CGAGTAA60400.054.4321481
CGTGTAT69500.050.9492031
TGACTAG120600.044.7749521
TATGTCG44650.043.1308061
CGTGTAA73700.042.8585971
TGACTAA145500.042.7285651
CGAGTAT60950.042.4941861
TTAGTCG26750.042.3378261
GATCTAA115650.042.063571
GATCTAG118600.041.9440161
ATACTCG64150.041.491821
ATACTAA156750.040.728921
TATGTAG139350.040.327781
CGAGTAG95100.040.2490841
AATGTCG55150.040.118021
AGACTAG115000.039.6003531
CTACTAG100600.038.856111
CGTGTAG97550.038.454431
CAACTAG91500.036.923451
Produced by FastQC (version 0.11.5)