FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32202219
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAA62600.054.73711
ATACTAG110050.053.1096421
CGTGTAT69150.046.926111
CGTGTAA75500.046.6506271
CGAGTAT70700.045.897321
TGACTAG124000.043.58911
TATGTCG48700.042.2946321
TGACTAA143850.041.6605151
ATACTCG66350.041.4155921
CGAGTAG93650.040.5691871
GATCTAG113200.040.3597451
CGTGTAG99300.039.5602951
GATCTAA120100.039.553081
TTAGTCG26050.039.0758861
ATACTAA156600.038.726371
CTACTAG98100.038.680181
GATCTAT97000.037.8871231
TATGTAG137850.037.5802351
AATGTCG55750.035.403171
GAACTAG120500.035.1385351
Produced by FastQC (version 0.11.5)