FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32583972
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAG111050.052.667371
CGAGTAA60000.052.482311
CGTGTAT76400.050.285351
CGAGTAT71650.046.549821
TGACTAG123750.044.2891121
CGTGTAA74500.043.614551
TATGTCG45800.042.8800351
GATCTAG118050.041.529831
CGAGTAG90200.041.1087231
TGACTAA143200.040.9764331
ATACTCG67050.040.0512731
GATCTAA124400.039.8324661
TATGTAG139950.039.1624031
TTAGTCG26550.038.8747861
ATACTAA162700.038.502941
CTACTAG97900.037.924261
CGTGTAG96600.037.6926461
GATCTAT96000.037.4304771
AATGTCG59550.037.2318841
CCGTAAA37050.035.3379331
Produced by FastQC (version 0.11.5)