FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56611189
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAG185500.060.414781
TGACTAG220250.051.577471
CGAGTAA112600.049.297321
CGTGTAT120750.046.4847451
ATACTAA239500.044.51731
GATCTAG207050.044.382081
TATGTAG211800.044.3348271
GATCTAA180900.043.5031551
TTAGTCG50050.042.1273351
ATACTTG329850.042.02051
GATCTAT143450.041.7285461
CTACTAG158750.041.5016141
CGTGTAA123100.041.286181
CAACTAG160200.041.0662841
TATGTCG81750.041.0562131
TGACTAA287750.040.575121
ATACTCG133650.040.102011
GAACTAG222850.038.8539621
GCCGTAT1408000.038.08122368-69
AATGTCG99450.038.0277981
Produced by FastQC (version 0.11.5)