FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15536189
Sequences flagged as poor quality0
Sequence length42
%GC32

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3178652.0459650690397755No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1305600.8403605285697798No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA609110.3920588247220731No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370380.23839823266825602No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA266720.17167659327522342No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA229550.14775180708731078No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA208920.13447313237499878No Hit
ACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA159130.10242537600437275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAAAG1733200.035.58680310
CCAAAGT1553700.035.37878411
GTCCAAA1706400.035.3774649
CAAAGTT1266400.035.368712
GGTCCAA629150.035.144478
AAAGTTT1198050.035.08406413
CGTCCAA330100.034.9939738
TGTCCAA472100.034.9656878
ACCGCTA109750.034.921539
AGTCCAA329250.034.827358
CACTCAC106000.034.81539
AAGTTTT1311300.034.7390614
ACCAGTT155100.034.5532729
ATACGCT78150.034.50715611
TCGACTC87800.034.4868559
GTCTCAC119850.034.4419869
CGAATGC108600.034.4293489
CGACTCA87600.034.42196310
CCAGTTC158650.034.41575610
CCAAAGG190250.034.4147811
Produced by FastQC (version 0.11.5)