FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25463128
Sequences flagged as poor quality0
Sequence length42
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21207828.328835326123327No Hit
TCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACC1175600.4616871894136494No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790000.31025253456684504No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470460.18476127520546573No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT456590.17931418323781745No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT437590.17185241341912116No Hit
TCTTTTAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACC312190.12260473261572577No Hit
TCTTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAAC273820.10753588482923229No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262580.10312165889438249No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG258680.10159003245791326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAACC605750.027.90863236
ACGACGC11050.021.6622610
TCATAAC794750.021.40908235
GCCGGGG32800.021.07548136
GCGAGTT16700.020.9105836
CGACGCT12350.020.1106311
GCGACAA23150.020.06267536
TTGCGAG17000.020.0120724
TGAGTTG156650.019.9679627
CACGACG11900.019.9637159
TGCGAGT18850.018.811965
GACGCTC13450.017.79683912
GCACGAC8300.017.7807888
ATCGCGA11050.017.75373319
CGAGTTG27950.017.7078277
CGGCCGG39150.017.52036934
ACGGCCG38750.017.51555433
TCGCGAA11000.017.50736620
GCGGACT54900.017.41174536
CTCATAA992650.017.18072134
Produced by FastQC (version 0.11.5)