FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28499989
Sequences flagged as poor quality0
Sequence length38
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5531531.940888468413093No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2944571.0331828549126807No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1245340.43696157216060677No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA503330.176607085707998No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA459460.1612140973107042No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390310.13695093005123615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCAC283550.030.9361729
ATGCACA281150.030.77396610
CCGAACA213050.030.67576210
CAATACG169400.030.5330589
CCCTAGA140750.030.51973510
CGAACAT212300.030.43817511
TCCGAAC215750.030.424879
CCTCTCA217050.030.4121729
TGCACAT281000.030.38684811
CACTATC153500.030.3293179
TACGCTG187900.030.22640210
GAACATT212300.030.21347212
CTACGCT189250.030.2131989
CCACAAC116550.030.1987119
CCTACAC99750.030.1845519
CCAATCG112000.030.15470710
CGACTTA119550.030.1233949
CCCAACT150100.030.10085111
AATACGC170700.030.0854410
ACAAGGG170500.030.07381610
Produced by FastQC (version 0.11.5)