FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31662652
Sequences flagged as poor quality0
Sequence length42
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25747648.131864633448897No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1225480.3870427530833488No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG671530.2120889936825254No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT598660.189074496981491No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT587970.18569827947450518No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT482480.15238142401969362No Hit
TCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACC477850.15091913336886625No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350550.11071403620896948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC13200.025.22626510
CGACGCT13250.024.72354111
CATAACC262600.024.00157236
CACGACG14000.023.3990679
GACGCTC17200.019.25510612
TCATAAC341550.018.68983535
GCCGGGG44400.018.08398636
GCGACAA22500.017.9228836
CCGATCT15300.017.17600321
ACGGCCG45950.017.15815433
GCACGAC10950.016.930838
ACGCTCT19850.016.8658813
TCGCGAA11500.015.96478220
GACGGCC50200.015.81266732
ATCGCGA11700.015.6918819
GCTCATC404350.015.68254523
TATGCTC416950.015.39854820
ATCCCTA414800.015.20918127
GCGGACT25250.015.18659836
CGGACTA42350.015.13348236
Produced by FastQC (version 0.11.5)