FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31662652
Sequences flagged as poor quality0
Sequence length42
%GC20

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5855081.849207072105015No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2588890.8176478710627272No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1861070.5877808340248947No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA781060.24668180037477594No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA717500.22660767645110713No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA473510.149548433277162No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN419770.13257575518310974No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA353640.11168994940790176No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340670.10759364060849988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAGA171400.034.7964110
CACGTGT310250.034.7874511
CGATATC378150.034.69414510
ACCGCTA191900.034.6705179
TCACGTG311900.034.64897510
AAACCGT172200.034.60400811
CGACAAG217400.034.5943810
ATTGCTC325450.034.59435310
ACGATCC292100.034.5757759
CTGCTTC1064350.034.558999
CCATTTC325450.034.549579
GAGAACG275900.034.531719
CCTACAT263000.034.51593811
TTCACGT316700.034.5153739
ACGAATT239700.034.5149111
ACCCTAC253750.034.510559
CGCATAA153000.034.463719
CCACAAC263950.034.4519239
ATCCACG155200.034.40476610
CAGACTT212400.034.39498512
Produced by FastQC (version 0.11.5)