FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10463024
Sequences flagged as poor quality0
Sequence length63
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130936812.514240624890089No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT579680.5540272104890517No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430430.41138202492893067No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT427970.40903088820211064No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT404790.38687668115833435No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT364160.34804469530032617No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251010.23990196333297142No Hit
TCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAA208930.19968414485143107No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134230.12828987107360168No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131170.12536528636463035No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124740.11921983548924288No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109610.10475938887266242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACG8300.040.1739589
CGACGCT8750.038.1078711
GACGCTC9250.036.04781312
ACGACGC9350.035.66244510
CCGCTAG20400.033.8083657
GCACGAC5550.032.8642588
GCGAGTT5550.031.3310816
ACGCTCT10950.030.71146813
CGCACGA1151.2732926E-1129.7385837
GACGCGA15650.026.76973244
GCCGCTA26550.025.76223856
ACGCGAT16450.025.6411145
GCTCATC224250.024.98501623
GCGCCGC27500.024.97590354
TCGCGAA17150.024.92629620
TATGCTC228450.024.86261220
CATCGCG17400.024.73194718
TGACGCG17000.024.64354543
ATCGCGA17350.024.63896219
GTTGACG17300.024.3808241
Produced by FastQC (version 0.11.5)