FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29346292
Sequences flagged as poor quality0
Sequence length42
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11509353.921909452819457No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1375560.46873383526613854No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT408740.13928165098336784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGGG51050.021.33182536
GCACGTC25650.021.051329
CATAACC144500.019.61919236
ATCGCGA36050.018.42489419
TCGCGAA36450.018.32084720
CGGCCGG59100.018.24307834
GCGACAA24800.018.2175736
CATCGCG37150.017.97612418
CGGCGGG37650.017.97534433
CGCGGCG36450.017.97507931
CGCGAAG37450.017.83233621
ACGGCCG61350.017.5151633
CCGCGGC37700.017.37923630
AGCATCG39000.016.93850116
CGAAGGC39700.016.82157323
GCATCGC39850.016.66765817
TCATAAC174750.016.33607735
GCGAAGG41350.016.32449222
GCGGCGG42400.016.08944332
GGCCCGC41650.015.99051727
Produced by FastQC (version 0.11.5)