FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29346292
Sequences flagged as poor quality0
Sequence length42
%GC22

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4713121.60603595166299No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2017130.6873543001616694No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA735170.25051546546323467No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA329980.11244350734327865No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320150.10909385076656361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA576400.035.2839169
GTATCAG576050.034.7272910
TATCAGT573100.034.54356411
CCTCATT327050.034.4715912
GATCCTG276400.034.282869
CAAGTTT454800.034.246413
AACACGC110550.034.2436269
CGACTTT284200.034.22867613
CTTACGA193800.034.2188229
TCCCTCA220150.034.16759510
CCTGATT279500.034.16717512
TTACGAC193250.034.07100310
CGACAAG197350.034.02897310
CCCTAGA129700.033.97286210
CCTACAT196900.033.96834611
ACCCTAC194750.033.9409839
ATCCACG86050.033.9281910
TCCTGAT281600.033.85008211
CAACGAC93750.033.84868210
ATCCTGA280600.033.84369710
Produced by FastQC (version 0.11.5)