FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23786654
Sequences flagged as poor quality0
Sequence length42
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11842364.978573278948775No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367340.15443113604797043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGGG50950.020.10121536
TCGCGAA23700.019.9735520
ATCGCGA24000.019.7238819
CATCGCG24600.019.3161818
CGCGAAG25100.018.93107621
CGCGGCG25250.018.8190931
CGGCGGG26150.018.58430533
CCGCGGC26200.018.27407830
AGGGGGG50800.018.1157441
AGCATCG26650.017.89823516
GCATCGC26550.017.89770317
CGAAGGC26900.017.73118823
CATAACC80600.017.59727536
CGGCCGG56650.017.47499334
GCGACAA22750.017.32674436
ACGGCCG57350.017.23031433
GGCCCGC28450.017.14489727
GCGGCGG28900.017.06502732
GCGAAGG28350.017.014822
GCACGTC13500.016.7996129
Produced by FastQC (version 0.11.5)