FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23786654
Sequences flagged as poor quality0
Sequence length42
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5224502.196399712208367No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1411310.593320102945122No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA534160.22456289985132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCAGAC162750.034.56091710
CAACCTT166100.034.49296612
CGAACTT143050.034.4008111
CAACGAC104200.034.3749110
CGAACTG120000.034.288139
CGACTTT223450.034.25182313
GTCCACT131950.034.11633311
CCACTTT240350.034.10513313
ACGATAG106300.034.0677649
CAATACG159300.034.0659459
CCAACGT101250.034.042599
GTCACTT166950.034.00468412
CGACTCA79700.033.98868610
ATCCACG108700.033.9784910
CCCTAGA77500.033.9780810
CAGACTT247500.033.94111312
TGTCCAC133150.033.91686610
CACTCAC97050.033.8837859
CCAAGGA133950.033.87554610
ACGTACT138950.033.873659
Produced by FastQC (version 0.11.5)