FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19245153
Sequences flagged as poor quality0
Sequence length42
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15924248.274415900980367No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1542440.8014693362011722No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT714140.37107525203878605No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT418850.21763921544297413No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379920.19741074544847734No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT364580.18943990728470697No Hit
GGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG289100.150219642317211No Hit
TCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACC287480.1493778719244269No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235550.12239445433351451No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGAC224550.1166787294442398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAACC166050.026.49762736
GCCGGGG74300.024.39974836
CGGCCGG87100.020.91541334
CGGCGGG52800.020.62596133
CGGGTGT53150.020.35709836
CGCGGCG53250.020.24830631
GCGACAA19500.020.12642136
CCGCGGC53600.019.98170330
ATCGCGA54800.019.97017719
TCGCGAA54800.019.93748720
CATCGCG55550.019.66809818
TCATAAC229700.019.46002835
GCATCGC56250.019.4552917
AGCATCG56900.019.39125816
CGCGAAG56750.019.31589521
GGCGGGT57350.019.14698834
CGAAGGC56800.019.14039423
TCGCCCC10900.018.9939836
GCGGCGG57650.018.98396132
GCGGGTG58000.018.87136535
Produced by FastQC (version 0.11.5)