FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26294149
Sequences flagged as poor quality0
Sequence length42
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15050545.723912190502914No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT651880.24791827261646687No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT407390.154935609439195No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT405530.15422822773233696No Hit
TCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACC378850.14408148367912574No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT375090.14265150775558472No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG308640.11737972580896229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC9250.026.46112310
CGACGCT9150.026.3568311
CATAACC270050.025.36567136
GCCGGGG59200.022.65533336
CACGACG11200.021.8540959
TCGCCCC13300.021.38657636
GACGCTC11850.021.11085912
CCGATCT10200.019.76311521
CGCGGCG23200.019.32273931
ATCGCGA23350.019.27006719
CGGCCGG68800.019.18136234
TCGCGAA23500.019.0706620
CCGCGGC23550.018.95850430
TCATAAC366000.018.83504135
GCACGAC7700.018.6986928
ACGGCCG70900.018.56236833
CATCGCG24500.018.43880518
GCGACAA15550.018.17628336
CGCACGA1401.4006218E-1017.997497
CGGCGGG25400.017.8619633
Produced by FastQC (version 0.11.5)