FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26333011
Sequences flagged as poor quality0
Sequence length42
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT638652624.252927247856313No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2762641.0491166391872164No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020080.7671283773815307No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1835340.6969730882655235No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1683290.639231875154725No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT938870.35653727558918347No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT671530.25501451391183483No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT583600.22162296594187425No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT554090.21041649965512868No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT469810.17841104460101428No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT467070.17737052553542015No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT452860.17197425695071483No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333110.12649901676644573No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324740.12332049684709431No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315510.11981539065168052No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302130.11473431579852376No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276060.10483419461602776No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274410.10420760466776852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC7500.029.27790610
CGACGCT7500.029.27773911
CACGACG8400.026.9980189
GCCGGGG69050.024.63377836
GCACGAC5600.024.4266858
CGGTACG2550.021.8806217
TCGCCCC16700.021.44862736
GACGCTC10650.020.78724512
CGGCCGG83200.020.29343834
CCTCACG24650.020.0806936
CGGCGGG70150.019.7064333
CGCGGCG70300.019.53617331
CCGCGGC70700.019.42582930
TCGCGAA71600.019.40742920
CATCGCG72250.019.3073118
GCCCGCG71700.019.22988128
CGCGAAG72050.019.21134421
GCATCGC72800.019.16133517
ACGGCCG88000.019.12508633
AGCATCG73200.019.0808216
Produced by FastQC (version 0.11.5)