FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23149410
Sequences flagged as poor quality0
Sequence length42
%GC29

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7967263.4416687077554027No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2738321.1828897583134947No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1103620.4767378520662082No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1044410.4511605263373883No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA714520.308655814554237No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA524650.22663644559407775No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA469210.20268767108967356No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA465330.20101160245552693No Hit
ACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA349680.15105352577020323No Hit
TCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA277860.12002897698040683No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA266650.11518652095236985No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA259460.11208061026177342No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA232870.10059435640044388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTACT170950.034.9187410
CAAGAGA223050.034.8816959
CCGATTC202300.034.820029
CGATATC193950.034.8060410
ATCGACG173200.034.7978679
AGAGACT202850.034.7874311
GTAATCT318050.034.76367611
TCGAAGT231800.034.73735411
CTCACAT347450.034.72867211
ATGTGCT241350.034.7128611
AAGAGAC223800.034.69225710
CTGACTG165250.034.663619
TCCGTAA167400.034.62689610
CCTAGTA168950.034.6075610
GAACAGC151150.034.5981649
CTGCCTA137050.034.58477810
CAGAAAG199200.034.5664910
ATCGAAG251250.034.56323610
CGGTAAT344900.034.562979
TCGACGA174100.034.54545210
Produced by FastQC (version 0.11.5)