FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22096965
Sequences flagged as poor quality0
Sequence length42
%GC19

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9630794.358422072895531No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3758731.7010164065517597No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3139001.4205570765034927No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1789490.8098351968245413No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA982160.44447732980524696No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA788910.3570218806066806No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA639800.2895420253414892No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA622870.281880339675607No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA615510.2785495655172554No Hit
TCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA416330.18841048985686495No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA408740.18497562900606487No Hit
CCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA357770.1619091128578065No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA312990.14164388638892264No Hit
GCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA285670.1292801975293892No Hit
ACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA278520.12604445904675143No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA264090.11951415047270066No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA248560.11248603597824407No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230660.1043853760007313No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA227340.10288290722277924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATCA459700.035.4868289
GATCACT440400.035.3682611
TGATCAC453200.035.24609410
ATCACTT435750.035.1888712
ACCCATC205900.035.169279
CGAACTG195200.035.096089
CCTACAC198350.035.0922859
CTACCGT173750.035.0673989
AACCGGA173850.035.057589
TCACTTT817900.035.0501813
CCAGATG183500.035.0384649
ACCGGAA174100.034.95649310
CTAATGG208200.034.9200310
ACGCTCT181850.034.891179
CGAATGC254200.034.8880549
ACGGTGA320350.034.87695310
GAATGCA254550.034.8763910
CTTCACT190600.034.86848411
TACGGTG322000.034.85949
CCGGAAT173950.034.8531411
Produced by FastQC (version 0.11.5)